Fig. 5.

Fluorescent microscopy results showing continuous stream purification of streptavidin-mNP conjugates. The magnet is located adjacent to the higher pH flowstream. The channel contains two fluidic streams flowing bottom to top. (a) No magnetic field. The conjugate aggregates largely remain in the left-hand stream and ca. 80% of the conjugates were isolated from the low pH outlet. (b) When the magnetic field is applied, the conjugate aggregates magnetophorese laterally across to the higher pH flowstream where they redissolve. When the magnetic field is applied, most conjugates were moved into the high pH stream and ca. 80% of the conjugates were collected from the right outlet.