Skip to main content
. 2008 Mar 28;29(5):1049–1056. doi: 10.1093/carcin/bgn078

Fig. 2.

Fig. 2.

(A) Effect of fisetin on cell cycle distribution in LNCaP cells. The cells treated with fisetin (10–60 μM, 48 h) were collected and stained with propidium iodide by using an apoptosis Apo-Direct Kit obtained from Phoenix Flow Systems as per vendor's protocol followed by flow cytometry. Following fluorescence-activated cell sorter analysis, cellular DNA histograms were further analyzed by ModiFitLT V3.0. The data are representative examples for duplicate tests. The details are described in Materials and methods. (B) Effect of fisetin on protein expression of cyclin D1, cyclin D2 and cyclin E in LNCaP cells. (C) Effect of fisetin on protein expression of cdk 2, cdk 4 and cdk 6 in LNCaP cells. (D) Effect of fisetin on protein expression of WAF1/p21 and KIP1/p27 in LNCaP cells. As detailed in Materials and methods, the cells were treated with fisetin (10–60 μM, 48 h) and then harvested. Total cell lysates were prepared and 40 μg protein was subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis followed by immunoblot analysis and chemiluminescence detection. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-actin. The immunoblots shown here are representative of three independent experiments with similar results. The values above the figures represent relative density of the bands normalized to β-actin.