Fig. 4.

(A) Effect of fisetin treatment of LNCaP cells on protein expression of Bax and Bcl-2. (B) Bax/Bcl-2 ratio. (C) Protein expressions of Bak, Bcl-xL, Bad and Bid. As detailed in Materials and methods, the cells were treated with fisetin (10–60 μM, 48 h) and then harvested. Total cell lysates were prepared and 40 μg protein was subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis followed by immunoblot analysis and chemiluminescence detection. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-actin. The immunoblots shown here are representative of three independent experiments with similar results. The values above the figures represent relative density of the bands normalized to β-actin.