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. 2003 Dec 1;31(23):6722–6732. doi: 10.1093/nar/gkg861

Figure 1.

Figure 1

The steady-state levels of C/EBPα mRNA and polypeptides are reduced in Hep3B cells following exposure to IL-6. (A) Hep3B cells were stimulated with IL-6 for the indicated period of time and total RNA was isolated and subjected to RT–PCR using primers for C/EBPα and GAPDH. The amplification products were size-fractionated by agarose gel electrophoresis along with molecular size markers (M). (B) and (C) Western blot analysis was carried out using whole cell extracts from Hep3B cells exposed to IL-6 for the indicated time and probed with antiserum against either C/EBPα, which recognises both the p42 and p30 isoforms (B) or C/EBPβ, which detects predominantly the p38 form (C).