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. 2003 Dec 1;31(23):7003–7010. doi: 10.1093/nar/gkg864

Figure 1.

Figure 1

EMSA and topology assay with different DNA sequences. (A) EMSA. A 175 ng aliquot of SV40 DNA (lanes 1–5), HK-plasmid (lanes 6–10) and Litmus-plasmid (lanes 11–15) wase incubated for 1 h at 37°C without (–) or with GST–DEK at molar ratios of DEK/DNA of 28, 56, 84 and 112. Nucleoprotein complexes were analyzed on 0.6% agarose gels and visualized by ethidium bromide staining. (B) Topology assay. A 175 ng aliquot of SV40 DNA (lanes 1 and 2), HK-plasmid (lanes 3 and 4) and Litmus-plasmid (lanes 5 and 6) was incubated without (–) or with GST–DEK (at a molar ratio of DEK/DNA of 84) and topoisomerase I for 1 h at 37°C. Deproteinized samples were analyzed by agarose gel electrophoresis and ethidium bromide staining.