Figure 2.

Progesterone receptor membrane component 1 (PGRMC1) protein expression. (A) Expression of PGRMC1 protein in healthy cycling women across an idealized 28-day menstrual cycle according to cycle phase and (B) in women with premature ovarian failure, polycystic ovary syndrome, and natural menopause. Western blots (example shown in figure 1) were analyzed using Odyssey infrared imaging system to determine integrated intensities for PGRMC1 and β-actin in the same sample. β-actin was used for normalization. Measured PGRMC1 levels were displayed as box plots (***p-value < 0.01; *p-value < 0.05; student's t-test). [Menstrual cycle analysis (HF: healthy women): eFP, lFP: early and late follicular phase; PO: preovulatory phase; eLP, mLP, lLP: early, mid- and late luteal phase; Study groups: HM: healthy men; PM: postmenopausal women; POF: premature ovarian failure; PCOS: polycystic ovary syndrome]