Table 2. Table showing β-actin dynamics changes upon dominant negative Rac1 expression.
t1/2 (seconds) | ||
Fast | Slow | |
Control | 19.5±2.3 | 445.7±65.2 |
ET-1 | 10.1±2.8* | 176.9±15.7* |
N17 | 61.8±10.5* | 1484±197.7* |
ET-1 + N17 | 15.3±2.9# | 621.8±106.2# |
FRAP experiments were performed in adult cardiomyocytes co-expressing the β-actin-GFP and N17 Rac following their treatment with +/− 100 nM ET. Analyses were made in at least 10 independent cells for statistical evaluation. Values are expressed as Mean ± S.D. *p<0.05 when compared to control. #p<0.05 when compared to ET-1 treated cells.