. 2003 Dec 1;31(23):6996–7002. doi: 10.1093/nar/gkg883

Table 1. Primers used for site-directed mutagenesis of the Baph_ and Hin_rpsA TIRs.

Name	Sequence (5′→3′)
SDbaph	GAAGATTATTAATATGAATGAATCTTTTGC^a
cSDbaph	GCAAAAGATTCATTCATATTAATAATCTTC^a
Eco-ss1-HinII	*TATGTTAAAC*AACCCCGCATTTTATGG^b
cEco-ss1-HinI	*GTTTAACATA*AGGATAAATGCTTATTC ^c
cHinII-ss1-HinI	GCGGGGTTGTTTAACATAAGG ^d
DSlac	GGCGATTAAGTTGGGTAACGCCAGGG^e
UPlac	GTTAGCTCACTCATTAGGCACCCC^f

^aAn initiator codon of the Baph_rpsA TIR is in bold; the base mutated is underlined.

^bThe Eco-ss1 region (bold italic) is followed by the Hin sequence covering the left part of the central hairpin II.

^cThe primer is complementary to the Eco-ss1 (bold italic) and the right part of the Hin hairpin I including the apical loop.

^dThe primer is complementary to the left part of the Hin hairpin II, Eco-ss1 (in bold italic) and the right part of the Hin hairpin I.

^eThe primer is complementary to the region (+57 to +82) of the genuine lacZ mRNA (+1 is A of the lacZ AUG start codon).

^cThe primer covers the positions from –64 to –41 with respect to the transcription start point of the lac-operon.