Table 1. Tm values for two transitions for both HPA11 and HPG11 oligonucleotides in 20 mM sodium cacodylate (pH 7.4) and 0.1 mM EDTA at different salt concentrations.
NaCl (mM) | Tm (°C) | |||
---|---|---|---|---|
HPA11 | HPG11 | |||
I | II | I | II | |
5 | 26 | 70 | 42 | 85 |
50 | 30 | 72 | 44 | 90 |
100 | 38 | 82 | 54 | >90 |
500 | 40 | 85 | 60 | >90 |