Figure 4.

Verification of the critical endpoints converged by PASA-PCR. A series of truncated Spc34p mutants (listed in the figure) were prepared, and their interaction with Duo1p (upper panels) and Dam1p (lower panels) were analyzed using two-hybrid analysis. The growth rate on the selection plate and β-galactosidase activity of each truncation mutant is summarized and shown as a list. The constructs having the critical endpoints converged from Duo1p and Dam1p interacting clones are highlighted in blue and green, respectively. The value in β-galactosidase activity is the percentage of the β-galactosidase activity of each clone toward that obtained using full-length of Spc34p [also included in the list as Spc34p (FL)].