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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Arthritis Rheum. 2010 Jul;62(7):2004–2012. doi: 10.1002/art.27475

Figure 2.

Figure 2

TLR2/TLR4- and MyD88-dependent pro-catabolic responses to exogenous LMW-HA. Mouse femoral head cartilages isolated from TLR2/TLR4−/−, TLR2−/−, TLR4−/− and congenic WT mice (A, B, E), or immature mouse chondrocytes isolated from MyD88−/−, TLR2/TLR4−/− and respective congenic WT mice (C, D) were stimulated with 100 µg/ml LMW-HA for 5 and 3 days, respectively. Conditioned media were analyzed for release of NO (n=8 for explants, n=3 for chondrocytes), MMP-3 and MMP-13 (all data shown were representative of 3 individual experiments) by Griess reaction and Western blot analyses. Statistics for NO release in A, #, ## p<0.008 for LMW-HA treated MyD88−/− and TLR2/TLR4−/− relative to WT mice explants, respectively. Statistics for NO release in B, * and ** p<0.01 for LMW-HA treated MyD88−/− and TLR2/TLR4−/− relative to WT mouse chondrocytes, respectively. Statistics for NO release in E, *p<0.05 and **p<0.004 for LMW-HA treated TLR2−/− and TLR4−/− relative to WT mice explants, respectively.