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. Author manuscript; available in PMC: 2011 Jul 23.
Published in final edited form as: J Mol Biol. 2010 May 19;400(4):702–714. doi: 10.1016/j.jmb.2010.05.022

Figure 2. Effect of Ca2+ and NaCl on the interaction between CaM and MA monitored by SDS-PAGE pulldown assays.

Figure 2

Molecular mass marker sizes (Mr) are shown along the left edge. a) Interactions between CaM and MA in high calcium buffer. Lane 1: molecular mass standards; lane 2: CaM-agarose beads only; lanes 3–7: CaM-agarose + MA in 0, 50, 100, 150, or 500 mM NaCl; lane 8: MA alone. b) Interactions between CaM and MA in low calcium buffer. Lane 1: CaM-agarose beads only; lane 2: molecular mass standards; lanes 3–7: CaM-agarose + MA in 0, 50, 100, 150, or 500 mM NaCl; lane 8: MA only.