. Author manuscript; available in PMC: 2011 Jul 23.

Published in final edited form as: J Mol Biol. 2010 May 19;400(4):702–714. doi: 10.1016/j.jmb.2010.05.022

Table 3. Secondary structure content of Ca²⁺-CaM, MA, and their complex derived from circular dichroism data.

Values for secondary structure content are the average results from CDpro and the quoted errors are the standard deviations between predictions calculated by three different algorithms. The stated values for helix and sheet are the summed from the amounts of ‘regular’ and ‘distorted’ versions of each structure calculated by CDpro. For comparison, the secondary structure content expected for an unaltered CaM-MA complex and the ‘MA residual’ spectrum generated from subtracting the Ca²⁺-CaM spectrum from the Ca²⁺-CaM-MA spectrum is also given below.

Spectrum	Helix (%)	Sheet (%)	Turn (%)	Undefined (%)
Ca²⁺-CaM	60 ± 5	6 ± 3	13 ± 6	21 ± 4
MA	53 ± 3	8 ± 1	17 ± 1	22 ± 5
Ca²⁺-CaM-MA	49 ± 2	6 ± 3	14 ± 1	32 ± 4
Expected complex	57 ± 4	69 ± 2	14 ± 4	22 ± 4
MA residual	41 ± 2	8 ± 1	15 ± 2	37 ± 2

Table 3. Secondary structure content of Ca2+-CaM, MA, and their complex derived from circular dichroism data.

Table 3. Secondary structure content of Ca²⁺-CaM, MA, and their complex derived from circular dichroism data.