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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Gastroenterology. 2010 Mar 17;139(1):194–203.e1. doi: 10.1053/j.gastro.2010.03.035

Figure 5.

Figure 5

Acid and bile salts increase CDX2 promoter activity by activating NF-κB in NES-B3T and NES-B10T cells. (5A &5B) NF-κB inhibition by Bay 11-705 and site-directed mutagenesis of the NF-κB binding site within the reporter construct pGL-3-CDX2 prevents activation of the CDX2 promoter in both cell lines. (5C &5D) NF-κB inhibition by infection with the adenoviral construct Ad5IκBα-SR prevents activation of the CDX2 promoter; the corresponding empty adenoviral vector served as a control. The bar graphs depict the mean + SEM of at least 3 individual experiments. (*, p<0.05 compared with non-treated controls; **, p<0.01 compared with non-treated controls; ***, p<0.001 compared with non-treated controls) RLU, relative light units.