Skip to main content
. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Gastroenterology. 2010 Mar 17;139(1):194–203.e1. doi: 10.1053/j.gastro.2010.03.035

Figure 6.

Figure 6

Acid and bile salts increase nuclear translocation of the NF-κB subunits p50 and p65 in NES-B3T cells. Representative experiments of (6A) immunofluoresence and (6B) Western blotting for p50 and p65. Note the prominent cytoplasmic and slight nuclear expression of both subunits in control cells. Following exposure to acid, bile salts, or acidified bile salts, there was strong nuclear localization of p50 and p65. DAPI staining demonstrates the total number of cell nuclei in the same field. Tubulin and lamin served as controls for the cytoplasmic and nuclear fractions, respectively. C, untreated control cells; A, acidic medium; B, bile salt medium; A&B, acidified bile salt medium.