Relative quantification of macrophage mRNA of genes encoding (A) proinflammatory and chemokine mediators TNF-α, IL-1 and IL-8 and (B) immune related and anti-viral molecules (iNOS and MX1) from BTV infected bMDM, as measured by real time quantitative PCR. Values are the means ±SD of 5 replicate experiments (5 individual cows) performed in duplicate. Expression of genes encoding TNF-α, IL-1β, IL-8, iNOS, and MX-1 was significantly increased (p < 0.05). (*) indicates genes with significant increase in mRNA expression.