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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Arch Biochem Biophys. 2010 Mar 25;500(1):66–73. doi: 10.1016/j.abb.2010.03.012

Figure 2.

Figure 2

Binding of NHG to the R48A/W191F CcP mutant. Optical difference spectra for R48A/W191F (5 μM) in 100 mM KPi pH 6.0, 2 ml volume upon successive additions of 5 μl of 500 mM NHG (corrected to pH 6.0).