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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Arch Biochem Biophys. 2010 Mar 25;500(1):66–73. doi: 10.1016/j.abb.2010.03.012

Figure 3.

Figure 3

Formation and decay of Compound I following reaction of R48A/W191F CcP with H2O2 by rapid-scan stopped flow mixing of 5 μM protein against 20 μM H2O2 in 100 mM KPi pH6, 25C. Data analysis by single value decomposition using a model for sequential conversion of three species, A, B and C, produced the spectra and time courses shown in the lower and upper panels respectively, and represent the ferric, Compound I and Compound II states. Pseudo-first order rate constants for the formation of Compound I are plotted as a function of H2O2 (inset) to give a bimolecular rate of 2.96 × 106 M−1sec−1.