Figure 4.
Effect of NHG on the decay of R48A/W191F Compound I. Compound I was formed as described in Figure 3 in the presence of 20 (red), 40 (blue), 80 (magenta), 160 (green) and 320 (orange) μM NHG. Shown in the inset are the substrate dependent rates for conversion of Compound I to Compound II for R48A/W191F (blue) and WT CcP (magenta) to give bimolecular rate constants of 1.73 × 105 M−1sec−1 and 2.8 × 101 M−1sec−1 respectively.
