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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Arch Biochem Biophys. 2010 Mar 25;500(1):66–73. doi: 10.1016/j.abb.2010.03.012

Figure 7.

Figure 7

Kinetics for the reaction of NHG with the Fe+2O2 complex of R48A/W191F. Absorbance at 408 nm representing the recovery of the Fe+3 state following photolysis of the Fe+2CO complex in the presence of O2 and 50 μM NHG. Fit to a single exponential (solid curve) gave kobs which is shown as a function of NHG and guanidine concentration (inset) to give the bimolecular rate constants reported in the text.