Figure 7.
Kinetics for the reaction of NHG with the Fe+2O2 complex of R48A/W191F. Absorbance at 408 nm representing the recovery of the Fe+3 state following photolysis of the Fe+2CO complex in the presence of O2 and 50 μM NHG. Fit to a single exponential (solid curve) gave kobs which is shown as a function of NHG and guanidine concentration (inset) to give the bimolecular rate constants reported in the text.
