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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Arch Biochem Biophys. 2010 Mar 25;500(1):66–73. doi: 10.1016/j.abb.2010.03.012

Figure 8.

Figure 8

Analysis for total nitrate and nitrite formed in reactions of WT, W191F, and R48A/W191F with NHG. Samples of enzyme (5 μM) were converted to the Fe+2O2 or Fe+2CO complexes as described in Methods and Figure 6 and incubated in the presence or absence of 200 μM NHG for 30 min before removal of protein by ultrafiltration and analysis of the filtrate for total nitrate and nitrite using nitrate reductase and the Griess reaction. Total product measured by this assay was normalized using a standard curve for nitrate.