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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cell Death Differ. 2010 May 21;17(8):1345–1353. doi: 10.1038/cdd.2010.57

Figure 5. A threshold of Sox2 is required to maintain osteoblast survival.

Figure 5

(A) Western Blot for Sox2 expression. OB1-Apert cells were infected with pBabe-Hygro retrovirus expressing the indicated shRNAs against Sox2 or scrambled (sc) shRNA. Pools of cells were selected in Hygromycin B and protein was extracted. Sox2 expression was determined by immunoblotting.

(B) Colony formation by Sox2 shRNA-expressing osteoblasts. OB5, OB1 and OB1-Apert cells infected with pBabe-Hygro retro-virus expressing either scrambled (sc) or sh900, sh998 and sh1043 were plated in triplicate and selected in Hygromycin B for 7-10 days followed by staining with Crystal Violet. Pictures of representative plates of OB1 and OB1-Apert cells expressing shRNA are shown and quantified in adjoining Table

(C) Growth of Sox2 shRNA-expressing OB1-Apert osteoblasts. OB1-Apert cells (0.1 × 106 cells/well) stably expressing either scrambled (sc) or sh900, sh998 or sh1043 were plated in triplicate and growth of cells assessed by counting cells in a Z1 Beckman Coulter counter. * = p<0.05