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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Exp Cell Res. 2010 May 13;316(14):2260–2277. doi: 10.1016/j.yexcr.2010.05.006

Figure 2.

Figure 2

BRFs induced by over-expression of TM-agrin-GFP in COS7 cells resemble filopodia in protein localization. (A) TM-agrin-GFP visualized by immunofluorescence staining of GFP (green). (B) F-actin labeled with rhodamine-phalloidin (red) is found throughout the BRFs. The distal GFP-positive areas lacking F-actin staining (arrows) represent TM-agrin-GFP shed onto the substratum before the tips of BRFs retracted. (D, G) TM-agrin-Ds-Red (red) visualized by immunofluorescence staining of Ds-Red. (E, H) Over-expressed myo10-GFP and truncated myo10-GFP, visualized by immunofluorescence staining with anti-GFP (green) were localized to the tips of BRFs. (J, M) Over-expressed TM-agrin visualized by immunofluorescence staining of GFP (green), (K) Fascin labeled with fascin antibody (red) is found in BRFs, (N) Myosin II-B labeled with myosin II-B antibody (red) is absent from BRFs, but present in stress fibers (arrows). (C, F, I, L, O) Overlay images. Reference bar = 10 μm.