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. 2003 Dec 1;31(23):6828–6840. doi: 10.1093/nar/gkg895

Figure 7.

Figure 7

Characterization of ATPase activity of HpDnaB (Wt) and HpDnaB (Mut). (A) ATPase activity is enhanced with increasing amounts of HpDnaB (Wt). HpDnaB (Mut) did not show any ATPase activity. Release of radiolabeled inorganic phosphate (Pi) is also marked. (B) Analysis of DNA-dependent ATPase activity of HpDnaB. Helicase activity was measured in the presence of 0.9 pmol of HpDnaB and increasing amounts of 36mer oligonucleotides. Release of inorganic phosphate was quantitated and plotted against protein or oligo concentration. (C) Effect of divalent cations on helicase activity. Helicase assays were performed in the presence of various divalent cations (2 mM each) as indicated. Percent unwinding activity in the presence of nucleotides and divalent cations is also shown. 18mer oligos annealed to M13mp19 ssDNA were used as substrate.