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. Author manuscript; available in PMC: 2011 Jul 16.
Published in final edited form as: J Mol Biol. 2010 May 7;400(3):555–566. doi: 10.1016/j.jmb.2010.05.001

Figure 2.

Figure 2

Acid fuchsin inhibits amyloid formation by human IAPP. (a) Fluorescent detected thioflavin-T kinetic assays are displayed: IAPP alone (black); A 1:1 molar ratio of acid fuchsin and IAPP (blue) samples were 16 µM IAPP and 16 µM acid fuchsin; A 5:1 molar ratio of IAPP and acid fuchsin (red), samples were 16 µM IAPP and 3.2 µM acid fuchsin; A 10:1 molar ratio of IAPP and acid fuchsin (green), samples were 16 µM IAPP and 1.6 µM acid fuchsin. Peptide solutions contained 20 mM Tris-HCl buffer (pH 7.4) and 2% HFIP by volume, and were continually stirred at 25°C. (b-e) TEM studies confirm that acid fuchsin inhibits amyloid formation by IAPP. (b) Image of IAPP alone. (c) Image of a 1:1 mixture of IAPP and acid fuchsin. (d) Image of a 5:1 mixture of IAPP and acid fuchsin. (e) Image of a 10:1 of IAPP and acid fuchsin. Samples were those used for the kinetic experiments depicted in Figure a. Aliquots were removed from the kinetic experiments after 60 minutes. Scale bars represent 100 nm.