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. Author manuscript; available in PMC: 2011 Jul 16.
Published in final edited form as: J Mol Biol. 2010 May 7;400(3):555–566. doi: 10.1016/j.jmb.2010.05.001

Figure 5.

Figure 5

Acid fuchsin inhibits amyloid formation if it is added during the lag phase. (a) Fluorescent detected thioflavin-T kinetic assays are displayed: IAPP alone (black); A 1:1 molar ratio mixture of acid fuchsin and IAPP with acid fuchsin added at 5 minutes (red arrow); A 1:1 molar ratio mixture of IAPP and acid fuchsin with acid fuchsin added at 9 minutes (green arrow). (b) TEM image of IAPP alone at 5 minutes (c) Image of a 1:1 mixture of acid fuchsin and IAPP. Acid fuchsin was added at 5 minutes and the reaction allowed to proceed for an additional 55 minutes before an aliquot was removed for TEM analysis. (d) Image of IAPP alone at 9 minutes. (e) Image of a 1:1 mixture of acid fuchsin and IAPP. Acid fuchsin was added at 9 minutes and the reaction allowed to proceed for an additional 51 minutes before an aliquot was removed for TEM analysis. Samples were 16 µM IAPP and 16 µM acid fuchsin; Solutions contained 20 mM Tris-HCl buffer (pH 7.4) and 2% HFIP by volume, and were continually stirred at 25°C