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. Author manuscript; available in PMC: 2011 Jul 16.
Published in final edited form as: J Mol Biol. 2010 May 7;400(3):555–566. doi: 10.1016/j.jmb.2010.05.001

Figure 6.

Figure 6

Acid fuchsin is not cytotoxic and protects rat INS-1 beta cells from human IAPP induced toxicity. (a) Alamar blue cell viability assays. Data are plotted as percent cell viability relative to control cells treated with buffer only. Data represent a minimum of three independent experiments performed in triplicate and are plotted as mean +/− standard deviation. (b–d) Evaluation of cell morphology by light microscopy. (b) Transformed rat INS-1 beta cells treated with 30 µM IAPP show cell rounding and detachment from the cell culture substratum, indicative of apoptosis. By contrast, INS-1 cells treated with either (c) a 1:1 molar ratio of 30 µM IAPP plus 30 µM acid fuchsin or (d) 30 µM acid fuchsin alone show few signs of apoptosis. Bright field images were obtained immediately before Alamar blue assays.