Figure 3.

Increased xCD11b⁺Gr-1⁺ cells by CD137 stimulation suppress T cell proliferation. Total splenocytes (4×10⁵) of DO11.10 mice (A, C) or OT-I mice (B, D) were stimulated with OVA protein (A, C: 100µg/ml, B, D: 250µg/ml) and serially diluted naïve splenic CD11b⁺Gr-1⁺ cells or 3H3 induced CD11b⁺Gr-1⁺ cells (A, B: 5 days after 3H3 treatment; C, D: 25 days after 3H3 treatment) were added. Three days after culture, T cell proliferation was examined including an 18 hr pulse with 4µCi/ml [³H] thymidine.