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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Atherosclerosis. 2010 Apr 14;211(1):122–129. doi: 10.1016/j.atherosclerosis.2010.04.005

Figure 2. LPC-treated VSMCs produce calcium phosphate deposits in culture.

Figure 2

(A) von Kossa stain, 14d, 4X. Arrows indicate calcium phosphate ridges. (B) Alizarin Red S calcium stain, 14d, 4X. Arrows indicate calcium structures. Scale bar = 200μm (C) Total phosphate levels; n=3, p<0.05, phosphate (μg) normalized to total protein (μg) (D) Calcium incorporation assay; 45CaCl2, n=6,*p<0.01, #p<0.05. CPM was normalized to total protein (μg/μL) concentrations (CPM/μg total protein). Error bars represent ±SD.