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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2010 May 4;264(1):54–60. doi: 10.1016/j.cellimm.2010.04.011

Fig 2.

Fig 2

IgE influences APC to promote Th2 differentiation. APC from RAG-1−/− spleens and LN were sensitized with or without 5 ug/ml anti-DNP IgE on DNP-HSA coated plates. After 24 hours of sensitization, purified CD4+ OTII T cells to APC with OVAp. After priming, live cells were normalized for cell number and restimulated with anti-CD3. After 48 hours restimulation, the supernatant was collected and subjected to CBA for cytokine quantification (A). T cell differentiation was determined by a ratio of [IFN-γ]: [IL-4] (B). Data are representative of at leaset 3 experiments where ** P=0.0026 for untreated (media) vs. IgE crosslinked (DNP+ IgE).