Fig 4.

IgE mediated DC cytokine regulation requires FcγRIII. Bone marrow derived dendritic cells were derived from WT, FcεRγ−/− and FcγRIII−/− bone marrow with GM-CSF. BMDC were sensitized with or without 5 ug/ml anti-DNP IgE on DNP-HSA coated plates and stimulated with LPS. After 24 hours incubation, supernatant was collected and IL-12 p70 was measured by ELISA. Data are reported as mean ± SEM and representative of 2 experiments where ** P=0.0054 and * P=0.014.