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. 2003 Dec 1;31(23):6860–6872. doi: 10.1093/nar/gkg906

Figure 6.

Figure 6

Id proteins inhibit E47-mediated transactivation of the hSHP promoter. HepG2 cells were transfected with 200 ng of hSHP (–355 bp)/Luc (A) and hSHP (–243 bp)/Luc (B), and 200 ng of expression vectors for E47, Id-1, Id-2 and Id-3 as indicated. The luciferase activity was measured and normalized against β-galactosidase activity. (C) 32P-labeled DNA fragments containing E-boxes (E6,7) in the hSHP promoter were combined with in vitro transcribed and translated E47 and Id proteins as indicated. DNA–protein complexes were analyzed on a 4% polyacrylamide gel by autoradiography.