Skip to main content
. 2003 Dec 1;31(23):6882–6890. doi: 10.1093/nar/gkg907

Figure 1.

Figure 1

STAT5-dependent and -independent expression of Cis and c-Fos in Ba/F3 cells stimulated with IL-3. (A) Cis but not c-Fos is up-regulated in cells expressing a constitutively active form of STAT5A. Ba/F3-WT and Ba/F3-1*6 cells were stimulated with IL-3, and mRNAs levels for Cis and c-Fos analyzed by real-time PCR, as described in Materials and Methods. (B) Cis but not c-Fos induction is abrogated in STAT5 knock-down cells. Ba/F3-β cells were transfected with siRNA duplexes specific for both STAT5A and STAT5B isoforms (5AB) or with a non-specific control (ScI), as described in Materials and Methods. Thirty-one hours post-transfection, cells were stimulated with IL-3 and mRNAs levels analyzed as above. (C) STAT5 protein levels were monitored by western blot from STAT5 knock-down (5AB) and control (ScI) cells 31 h following siRNA transfection. As a control for specificity, STAT3 expression was shown to be unaffected following STAT5 knock-down at both the protein (this figure) and RNA (not shown) level.