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STAT5 is constitutively bound to the Cis promoter in cells expressing STAT5A-1*6, a constitutively active form of STAT5A. Unstimulated and IL-3-stimulated (30 min) Ba/F3-WT and Ba/F3-1*6 cells were analyzed by ChIP, using antibodies specific for STAT5A+B (STAT5) (A), acetylated histone H3 (Ac-H3) (B), acetylated histone H4 (Ac-H4) (C) or no antibody as a control (D), as described above. Amplicons specific for Cis were analyzed by real-time PCR as before.
