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. 2003 Dec 1;31(23):6882–6890. doi: 10.1093/nar/gkg907

Figure 5.

Figure 5

Chromatin is constitutively accessible to restriction enzyme digestion at position –184 of the Cis promoter in cells expressing STAT5A-1*6. (A) Structure of the Cis promoter, indicating the position of the restriction sites analyzed by CHART-PCR. Gray boxes represent STAT5 binding sites. (B) Nuclei from unstimulated and IL-3-stimulated (30 min) Ba/F3-WT and Ba/F3-1*6 cells were analyzed by CHART-PCR, as described in Materials and Methods. Purified genomic (naked) DNA was used as to control the cutting efficiency of the restriction enzyme.