Figure 5.

Frog Prince-mediated cut-and-paste transposition into human chromosomes. (A) Excision. On top, a schematic of the FP-neo element is shown. IRs are represented by black arrows, the SV40 promoter and the neomycin-resistance marker gene (neo) are indicated. pUC19 vector backbone sequences that flank the element in the donor construct are shown in italics. The transposon footprints are depicted in the white box. (B) Integration. Three regions of human genomic sequences that served as target sites for the transposase are illustrated below. Flanking TA target site duplications are typed in bold.