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Comparison of the three cysteine-specific probes used to resolve the effects of probe size and charge on receptor function. The 5-fluorescein-maleimide (5FM) probe is a bulky dianion, while the 5-tetramethylrhodamine-maleimide (5TRM) probe is a bulky zwitterion with no net charge, and the N-ethyl-maleimide (NEM) probe is smaller and nonionic.
