Figure 3.

Metal-dependent recruitment of c-fos and MTF-1 to the MT-I promoter is rapid and sustained. (A and B) ChIP was carried out as described in the text and in the legend for Figure 1. Hepa cells, cultured in DMEM medium containing 2% serum, remained untreated or were treated for the indicated times with zinc (100 µM) or cadmium (10 µM), and chromatin was isolated and immunoprecipitated using antibodies against the indicated transcription factors. (C) Fold-recruitment was calculated from triplicate PCRs and normalized to input samples.