Skip to main content
. 2003 Dec 1;31(23):6710–6721. doi: 10.1093/nar/gkg913

Figure 4.

Figure 4

Zinc-dependent recruitment of MTF-1 and c-fos to the MT-I promoter is reversible in the visceral yolk sac and in cultured cells. (A and B) Visceral yolk sacs were obtained on day 14 of pregnancy from mice that had been fed with a zinc-adequate (Dietary zinc A) or zinc-deficient (Dietary zinc D) diet for the previous 6 days. (A) Total RNA was extracted from yolk sacs and MT-I and actin mRNA levels were assayed by northern blotting. (B) Chromatin was isolated from yolk sacs, immunoprecipitated using antibodies against the indicated transcription factors or Sepharose beads only (no ab) and subjected to PCR using primers to the MT-I gene (Fig. 1). (C and D) Hepa cells remained untreated (0 h) or were treated with zinc (50 µM) for the indicated times. Alternatively, medium from cells that had been pretreated with zinc (50 µM) for 3 h was replaced with fresh medium containing 0.75 µM zinc (DMEM with 2% FBS), and cells were cultured for an additional 16 h (3 + MC). (C) RNA was extracted from Hepa cells and MT-I and actin mRNAs assayed by northern blotting. (D) ChIP carried out using antibodies against the indicated transcription factors.