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c-Fos is not required for induction of the MT-I gene by zinc in mouse embryo fibroblasts. Northern blot detection analysis of MT-I and actin mRNAs isolated from mouse embryonic fibroblasts treated with or without zinc (100 µM) for 3 h. The fibroblasts used were MEFs (wt) or fibroblasts with targeted deletions of the c-fos gene only, or both c-fos and fosB genes (c-fos/fosB), as indicated.
