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. 2008 Mar 26;23(6):1324–1337. doi: 10.1093/humrep/den088

Figure 1:

Figure 1:

The cDNA sequence and the corresponding amino acid (aa) sequence of single chain variable fragment (scFv) clone (AFA-1) reactive with FA-1 antigen

The aa sequence contains heavy chain (1–109 aa), linker (Gly4Ser)3 (110–124 aa) shown in shaded grey box, light chain (125–223 aa) and E-Tag (224–236 aa) sequence (shown after ↑ in italics). The aa sequence of immunoglobulin (Ig)G1 heavy chain includes framework region 1 (1–25 aa), framework region 2 (34–50 aa), framework region 3 (59–96 aa), and their corresponding CDR-1 (26–33 aa), CDR-2 (51–58 aa) and CDR-3 (97–109 aa) regions. Similarly, the aa sequence of Igк3 light chain includes framework region 1 (125–150 aa), framework region 2 (158–174 aa), framework region 3 (178–213 aa), and their corresponding CDR-1 (151–157 aa), CDR-2 (175–177 aa) and CDR-3 (214–223 aa) regions. All CDRs regions are enclosed in rectangular boxes.