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. 2008 Mar 26;23(6):1324–1337. doi: 10.1093/humrep/den088

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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

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Figure 10: — Epifluorescent photomicrographs indicating the indirect immunofluorescent reaction pattern of AFA-1, FAB-7 and YLP20 scFv antibodies with methanol-fixed (a, b and c) and unfixed live human sperm (a', b', and c’)

AFA-1 (a and a’) and FAB-7 (b and b’) antibodies predominantly reacted with post-acrosomal, midpiece and tail regions of methanol-fixed (a and b) and unfixed live human sperm (a' and b’). YLP20 antibody reacted with acrosomal, midpiece and tail regions of methanol-fixed (c) and unfixed live (c’) human sperm. Magnifications: a, b and c ×698; a', b' and c' ×1924.