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. 2008 Mar 26;23(6):1324–1337. doi: 10.1093/humrep/den088

Figure 9:

Figure 9:

Immunoreactivity pattern of AS-16 scFv antibody with HSE

The purified scFv antibody was examined for its reactivity with LIS-solubilized HSE (B) and MC-solubilized human sperm preparation (C), in the western blot procedure. (A) SDS–PAGE of LIS-solubilized human sperm preparation (lane a) and MC-solubilized human sperm preparation (lane a’) revealed several protein bands of various molecular identities after silver staining. (B) AS16 scFv antibody specifically recognized four protein bands of 18 (major band), 37, 55 and 100 kDa, respectively, on the western blot of LIS-solubilized human sperm preparation (lane b). Control scFv antibody did not react with any band on the western blot (lane b’). (C) AS-16 ScFv antibody recognized two protein bands of 18 (major band) and 100 kDa (minor band), in the western blot of MC-solubilized human sperm preparation (lane c). Control scFv antibody did not react with any specific band on the western blot (lane c’). The 18 kDa protein was the major band specifically recognized in both the LIS-solubilized human sperm preparation (lane a) and MC-solubilized human sperm preparation (lane a’) and it corresponds to SAGA-1 sperm protein.