Figure 2.

Multiple imaging and integration of a microarray. A microarray composed of 9600 features was hybridised with fluorescent cDNA target and analysed with a single confocal laser scan or with 10 consecutive scans followed by image overlay and integration with the software ΣPOT, according to the mean or to the maximum pixel principle. Note the general improvement of the signal across the entire microarray area. Significant reduction of background is achieved with the mean pixel calculation.