Skip to main content
. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: J Invest Dermatol. 2007 Nov 8;128(5):1286–1293. doi: 10.1038/sj.jid.5701155

Figure 2. IGF-1 does not activate p38 MAPK or SAPK/JNK pathways in SEB-1 cells.

Figure 2

a. A Western blot probing for phosphorylated MAPK/p38 reveals that SEB-1 sebocytes do not phosphorylate p38 in response to IGF-1. C-6 glioma cells treated with anisomycin serve as a positive control for phosphorylated p38, while unstimulated C-6 glioma cells represent the negative control. The phospho p38 blot was stripped and re-probed with an antibody that recognizes total p38 as a loading control.

b. A Western blot probing for phosphorylated SAPK/JNK reveals that SEB-1 sebocytes do not phosphorylate SAPK/JNK in response to IGF-1. Total cell extracts from 293 cells treated with UV light (Cell Signaling Technology) serve as a positive control for phosphorylated JNK, while untreated 293 extracts serve as the negative control. The phospho SAPK/JNK was stripped and re-probed with an antibody that recognizes total SAPK/JNK as a loading control.