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. 2009 Mar 24;2(4):700–710. doi: 10.1093/mp/ssp006

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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

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Figure 1. — Major Pericentromere Repeat Organization at Interphase Is Disrupted in nrpe1 and drd1 But Not nrpd1 Mutants.

DNA-FISH of 5S genes (red) and 180-bp centromeric repeats (green) (left panel).

Organization pattern of pericentromeric regions analyzed by FISH using the 180-bp centromere repeat (green) and the BAC F28D6 (red) DNA probes (middle panel).

Immunostaining followed by DNA-FISH performed respectively with an antibody recognizing the A. thaliana centromeric histone H3, HTR12 (red), and 180-bp centromeric repeats (green) (right panel). In all panels, DNA was visualized by DAPI (gray) and size bars indicate 5 μm.