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. 2009 Mar 24;2(4):700–710. doi: 10.1093/mp/ssp006

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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

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Figure 4. — Specific Centromere Sequences Are De-Repressed in pol V and drd1 Mutants.

(A) Strand-specific centromere transcription was evaluated by RT–PCR using primers specific for different subsets of 180-bp repeat families: forward conserved (CEN-Fc), reverse conserved (CEN-Rc), forward non-conserved (CEN-F), and reverse non-conserved (CEN-R) 180-bp repeats.

(B) Different centromere sequences are up-regulated in nrpe1. The figure displays aligned centromere transcript consensus sequences obtained by cloning RT–PCR products from the following mutants: nrpe1 CEN-Fc 180-bp (n = 12), nrpe1 CEN-F (n = 15), and met1 CEN-F 180-bp (n = 10).

(C) Transposable element transcription detected by RT–PCR.

(D) Northern blot analysis of centromere-derived siRNAs (siCEN). Ethidium bromide-stained 5S rRNA resolved by agarose gene electrophoresis serves as a loading control.