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. 2009 Mar 24;2(4):700–710. doi: 10.1093/mp/ssp006

Figure 5.

Figure 5.

Pericentromere-Repeat RNAs Localized by RNA-FISH during Interphase.

The probe specifically detects forward transcripts (CEN-F, red). RNase A treatment prior to in-situ hybridization eliminates the FISH signals, indicating that the method predominantly detects RNA (bottom nuclei). DNA was DAPI-counterstained (gray). The size bar corresponds to 5 μm.