Sequence alignments for human, rat, mouse, and Ciona intestinalis As3mt showing the presence of motifs I, I′, II, and III in the methyltransferase domain and the positions of conserved cysteine residues. Sequences of human, rat, and mouse As3mt from NCBI GenBank (Homo sapiens [NP_065733], Rattus norvegicus [NP_543166], Mus musculus [NP_065602], and C intestinalis [ENSCINP00000027827]) were aligned using M-Coffee software, a meta-method for assembling multiple sequence alignments (Wallace et al., 2006).