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. 2009 Oct 15;113(1):70–76. doi: 10.1093/toxsci/kfp250

FIG. 3.

FIG. 3.

End-point RT-PCR analysis of constitutive As3mt RNA expression in heart, branchial sac, and gastrointestinal tract of Ciona Intestinalis using four replicate animals (A). Steady-state As3mt RNA expression in the gastrointestinal tract was compared in six animals exposed to 1 ppm arsenite in seawater for 5 days to six animals maintained under similar conditions in control unamended seawater to examine the extent to which As3mt expression is modulated by exposure to iAs (B). The RNA isolation protocol, primer sequences, and reaction conditions are provided in the “Materials and Methods” section.